The involvement of jasmonates and ethylene in Alternaria alternata f. sp. lycopersici toxin-induced tomato cell death

• DOI: 10.1093/jxb/err217
• Publication Date: 2011-08-25T00:30:33Z
• Authors: Liping Zhang; Chengguo Jia; Lihong Liu; Zhiming Zhang; Chuanyou Li; Qiaomei Wang
• Keywords: Jasmonic acid

Previous studies have shown that an ethylene (ET)-dependent pathway is involved in the cell death signalling triggered by Alternaria alternata f. sp. lycopersici (AAL) toxin detached tomato (Solanum lycopersicum) leaves. In this study, role of jasmonic acid (JA) programmed (PCD) induced AAL was analysed using a 35S::prosystemin transgenic line (35S::prosys), JA-deficient mutant spr2, and JA-insensitive jai1. The results indicated JA biosynthesis play positive toxin-induced PCD process. addition, treatment with exogenous ET action inhibitor silver thiosulphate (STS) greatly suppressed necrotic lesions 35S::prosys leaves, although leaflets co-treated STS still significant high relative conductivity. Application 1-aminocyclopropane-1-carboxylic (ACC) markedly enhanced sensitivity spr2 jai1 mutants to toxin. However, compared alone, application ET-insensitive Never ripe (Nr) did not alter death. reduced ET-mediated gene expression leaves restored co-treatment ACC Furthermore, decreased biosynthetic genes but ET-responsive Nr alone. Based on these results, it proposed both promote JAI1 receptor-dependent also acts upstream toxin-triggered PCD.