Suppression of starch synthase I expression affects the granule morphology and granule size and fine structure of starch in wheat endosperm

• DOI: 10.1093/jxb/eru095
• Publication Date: 2014-03-15T03:24:25Z
• Authors: S. J. McMaugh; J. L. Thistleton; E. Anschaw; J. Luo; C. Konik-Rose; H. Wang; M. Huang; O. Larroque; A. Regina; S. A. Jobling; M. K. Morell; Z. Li
• Keywords: Starch synthase; Granule (geology); Degree of polymerization

Studies in Arabidopsis and rice suggest that manipulation of starch synthase I (SSI) expression wheat may lead to the production grains with novel structure properties. This work describes suppression SSI using RNAi technology, which leads a low level enzymatic activity for developing endosperm, abundance protein inside granules mature grains. The amylopectin fraction from suppressed lines showed an increased frequency very short chains (degree polymerization, dp 6 7), lower proportion (dp 8-12), more intermediate 13-20) than grain their negative segregant lines. In most severely affected line, amylose content was significantly increased, morphology changed, B reduced. change fine SSI-RNAi alters gelatinization temperature, swelling power, viscosity starch. demonstrates roles determination properties are similar among different cereals Arabidopsis.