The differential transcriptome and ontology profiles of floating and cumulus granulosa cells in stimulated human antral follicles

• DOI: 10.1093/molehr/gap103
• Publication Date: 2009-11-21T01:54:52Z
• Authors: S. Kõks; A. Velthut; A. Sarapik; S. Altmäe; E. Reinmaa; L.C. Schalkwyk; C. Fernandes; H.V. Lad; U. Soomets; Ü. Jaakma; A. Salumets
• Keywords: Granulosa cell; Antral follicle; Follicular fluid

Communication between various ovarian cell types is a prerequisite for folliculogenesis and ovulation. In antral follicles granulosa cells divide into two distinct populations of mural cumulus (CGC), enveloping the antrum surrounding oocyte, respectively. Both types, with compartment in excess, contribute to floating (FGC) population follicular fluid. The aim this study was compare transcriptomes FGC CGC stimulated obtained from 19 women undergoing IVF–ICSI procedure. were fluid during follicle puncture procedure acquired after oocyte denudation micromanipulation. Gene expression analysis conducted using genome-wide Affymetrix transcriptome array. profile varied significantly. Out 28 869 analysed transcripts 4480 differentially expressed (q-value < 10−4) 489 showed ≥2-fold difference level 222 genes up-regulated 267 CGC. higher involved immune response, hematological system function organismal injury, although had protein degradation nervous up-regulated. Cell-to-cell signalling interaction pathways noted both populations. Furthermore, numerous novel that have not been previously described physiology identified. conclusion, our results provide solid basis future studies biology will help identify molecular markers embryo viability IVF.