A new condensing reagent, 1-(2,4,6-triisopropylbenzenesulfonyl)-5-(pyridin-2-yl)tetrazolide and its use in the synthesis of λ cro binding heptadecanucleotide on a polymer support

• DOI: 10.1093/nar/10.20.6235
• Publication Date: 2007-01-04T17:15:07Z
• Authors: E. Ohtsuka; Z. Tozuka; S. Iwai; M. Ikehara
• Keywords: Azoles; Magnetic Resonance Spectroscopy; Polydeoxyribonucleotides; Base Sequence; Tetrazoles; Indicators and Reagents; Chromatography, Thin Layer; 01 natural sciences; 0104 chemical sciences

A new condensing reagent 1 -(2,4,6-triisoprophylbenzenesulfonyl)-5-(pyridin-2-y1)tetrazolide (TPSPy) was found to give one diastereoisomer of dinucleoside monophosphate aryl esters. Several oligodeoxynucleotide blocks were prepared using this reagent. heptadecanucleotide .dTATCCCTTGCGGTGATA, which had the same sequence as λ cro binding DNA synthesized by mono-, tri- and dodecanucleotide on a polystyrene support.