Nucleotide sequence of the human γ cytoskeletal actin mRNA: anomalous evolution of vertebrate non-muscle actin genes

• DOI: 10.1093/nar/14.13.5275
• Publication Date: 2007-01-04T23:41:04Z
• Authors: Harry P. Erba; Peter Gunning; Larry Kedes
• Keywords: Coding region; Conserved sequence

Two distinct, but iso-coding. γ non-muscle actin cDNAs were isolated from an SV40-transformed human fibroblast library. The complete nucleotide sequence of the eDNAs indicates that they may have arisen polymorphic alleles. By using genomic DNA and cellular RNA transfer blots, we demonstrate 3′ untranslated region (UTR) mRNA consists evolutionanly conserved 5′ more divergent segments. In fact, segment UTR detects a single- copy in chicken genome 20S transcript tissues. coding regions these compared with those other vertebrate genes. Surprisingly, percentage silent base substitutions between β is anomalously low greater conservation than would be expected for gene pair which arose during pre-avian evolution. We discuss conversion recent selective pressure as possible explanations apparently anomalous evolution gene.