Differential Expression Control and Polarized Distribution of Plasma Membrane-Resident SYP1 SNAREs in Arabidopsis thaliana

• DOI: 10.1093/pcp/pcn197
• Publication Date: 2008-12-20T01:34:48Z
• Authors: Kazuhiko Enami; Mie Ichikawa; Tomohiro Uemura; Natsumaro Kutsuna; Seiichiro Hasezawa; Tsuyoshi Nakagawa; Akihiko Nakano; Masa H. Sato
• Keywords: Root hair; Eukaryote; Lateral root

Membrane trafficking to the plasma membrane (PM) is a highly organized process which enables plant cells build up their bodies. SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) genes, encode proteins involved in trafficking, are much more abundant Arabidopsis genome than that of any other eukaryote. We have previously shown large number molecules cell localized predominantly on PM. In present study, order elucidate physiological function each PM-localized SNARE, we analyzed spatiotemporal expression profiling nine SYP1s resident PM Arabidopsis, and used information thus acquired generate transgenic plants expressing green fluorescent protein-fused Qa-SNAREs under control authentic promoters. Among SYP1s, only SYP132 expressed ubiquitously all tissues throughout development. The patterns contrast, tissue specific, different from one another. A particularly noteworthy example SYP123, root hair during development, shows focal accumulation pattern at tip region hairs. These results suggest constitutive while events such as formation or growth hair, with some redundancy.