Rare instances of haploid inducer DNA in potato dihaploids and ploidy-dependent genome instability

• DOI: 10.1093/plcell/koab100
• Publication Date: 2021-03-31T11:43:00Z
• Authors: Kirk R. Amundson; Benny Ordoñez; Monica Santayana; Mwaura Livingston...; Isabelle M. Henry; Merideth Bonierbale; Awais Khan; Ek Han Tan; Luca Comai
• Keywords: Parthenogenesis; Chromosome instability; Polyploid

In cultivated tetraploid potato (Solanum tuberosum), reduction to diploidy (dihaploidy) allows for hybridization diploids and introgression breeding may facilitate the production of inbreds. Pollination with haploid inducers (HIs) yields maternal dihaploids, as well triploid hybrids. Dihaploids result from parthenogenesis, entailing development embryos unfertilized eggs, or genome elimination, missegregation loss paternal chromosomes. A sign elimination is occasional persistence HI DNA in some dihaploids. We characterized genomes 919 putative dihaploids 134 hybrids produced by pollinating clones three HIs: IVP35, IVP101, PL-4. Whole-chromosome segmental aneuploidy was observed 76 karyotypes ranging 2n = 2x - 1 23 + 3 27. Of additional chromosomes 74 aneuploids, 66 were non-inducer parent 8 inducer parent. Overall, we detected full partial 0.87% irrespective parental genotypes. Chromosomal breaks commonly affected dihaploid progeny, but not correlating instability sperm ploidy induction. The residual discovered progeny consistent mechanism