Brassinosteroids regulate cotton fiber elongation by modulating very-long-chain fatty acid biosynthesis

• DOI: 10.1093/plcell/koad060
• Publication Date: 2023-03-02T13:19:51Z
• Authors: Zuoren Yang; Zhao Liu; Xiaoyang Ge; Lili Lu; Wenqiang Qin; Ghulam Qanmber; Le Liu; Zhi Wang; Fuguang Li
• Keywords: Elongation; Brassinosteroid; Crosstalk

Abstract Brassinosteroid (BR), a growth-promoting phytohormone, regulates many plant growth processes including cell development. However, the mechanism by which BR fiber is poorly understood. Cotton (Gossypium hirsutum) fibers are an ideal single-cell model in to study elongation due their length. Here we report that controls cotton modulating very-long-chain fatty acid (VLCFA) biosynthesis. deficiency reduces expression of 3-ketoacyl-CoA synthases (GhKCSs), rate-limiting enzymes involved VLCFA biosynthesis, leading lower saturated contents pagoda1 (pag1) mutant fibers. In vitro ovule culture experiments show acts upstream VLCFAs. Silencing BRI1-EMS-SUPPRESOR 1.4 (GhBES1.4), encoding master transcription factor signaling pathway, significantly length, whereas GhBES1.4 overexpression produces longer endogenous and directly binds RESPONSE ELEMENTS (BRREs) GhKCS10_At promoter region, turn increase contents. promotes elongation, silencing inhibits growth, supporting positive regulatory role for elongation. Overall, these results uncover through crosstalk between VLCFAs at level.