The transcription factor MYB30 promotes iron homeostasis by maintaining the stability of the FIT transcription factor

• DOI: 10.1093/plcell/koaf090
• Publication Date: 2025-04-17T17:07:16Z
• Authors: Hongyun Zhao; Juntao Jiang; Mengai Shen; Yiyi Zhang; Yamei Zhang; Huilin Liu; Huapeng Zhou; Yuan Zheng
• Keywords: MYB; Homeostasis; Transcription

Abstract Iron (Fe) is a vital nutrient for the growth and development of plants. In Arabidopsis (Arabidopsis thaliana), bHLH transcription factor FER-LIKE IRON DEFICIENCY INDUCED TRANSCRIPTION FACTOR (FIT) plays pivotal role in regulating response to Fe deficiency. Our study reveals that R2R3-MYB MYB30 positive regulator Fe-deficiency by FIT stability. Plants with loss-of-function mutations exhibit pronounced symptoms diminished uptake, while overexpression leads opposite effects. We have discovered interacts BRUTUS LIKE1 (BTSL1) BTSL2, two partially redundant E3 ubiquitin ligases negatively regulate response. binds C-terminal region BTSL1 through its MYB DNA-binding domain, thereby safeguarding from BTSL1-mediated ubiquitination degradation, resulting accumulation deficiency summary, our research uncovers as stability, which turn modulates homeostasis plants