A genetic approach reveals different modes of action of prefoldins

• DOI: 10.1093/plphys/kiab348
• Publication Date: 2021-07-22T19:10:48Z
• Authors: Noel Blanco-Touriñán; David Esteve-Bruna; Antonio Serrano-M...; Rosa María Esquin...; Francesca Resentini; Javier Forment; Cristian Carrasco...; Claudio Novella-R...; Alberto Palacios-...; Pedro Carrasco; Julio Salinas; Miguel Á Blázquez; David Alabadí
• Keywords: Chaperonin; Chaperone (clinical)

The prefoldin complex (PFDc) was identified in humans as a co-chaperone of the cytosolic chaperonin T-COMPLEX PROTEIN RING COMPLEX (TRiC)/CHAPERONIN CONTAINING TCP-1 (CCT). PFDc is conserved eukaryotes and composed subunits PFD1-6, PFDc-TRiC/CCT folds actin tubulins. PFDs also participate wide range cellular processes, both cytoplasm nucleus, their malfunction causes developmental alterations disease animals altered growth environmental responses yeast plants. Genetic analyses indicate that not all functions require canonical complex. lack systematic genetic plants animals, however, makes it difficult to discern whether process or alternative configurations, which necessary understand mode action. To tackle this question, on premise cannot be formed if one subunit missing, we generated an Arabidopsis (Arabidopsis thaliana) mutant deficient six compared various with those individual mutants. In way, demonstrate required for seed germination, delay flowering, respond high salt stress low temperature, whereas at least two redundantly attenuate response osmotic stress. A coexpression analysis differentially expressed genes sextuple several transcription factors, including ABA INSENSITIVE 5 (ABI5) PHYTOCHROME-INTERACTING FACTOR 4, acting downstream PFDs. Furthermore, transcriptomic allowed assigning additional roles PFDs, instance, higher temperature.