Single-cell transcriptomics sheds light on the identity and metabolism of developing leaf cells

• DOI: 10.1093/plphys/kiab489
• Publication Date: 2021-10-19T13:37:29Z
• Authors: Rubén Tenorio Berrío; Kevin Verstaen; Niels Vandamme; Julie Pevernagie; Ignacio Achon; Julie Van Duyse; Gert Van Isterdael; Yvan Saeys; Lieven De Veylder; Dirk Inzé; Marieke Dubois
• Keywords: Plant cell

Abstract As the main photosynthetic instruments of vascular plants, leaves are crucial and complex plant organs. A strict organization leaf mesophyll epidermal cell layers orchestrates photosynthesis gas exchange. In addition, water nutrients for growth transported through tissue. To establish single-cell transcriptomic landscape these different tissues, we performed high-throughput transcriptome sequencing individual cells isolated from young Arabidopsis (Arabidopsis thaliana) seedlings grown in two environmental conditions. The detection approximately 19,000 transcripts over 1,800 high-quality revealed 14 populations composing young, differentiating leaf. Besides comprising core identified subpopulations with a distinct identity or metabolic activity. proposed cell-type-specific markers each populations. Finally, an intuitive web tool allows browsing presented dataset. Our data present insights on how constituting developing connected via developmental, metabolic, stress-related trajectories.