Re enhances anthocyanin and proanthocyanidin accumulation to produce red foliated cotton and brown fiber

• DOI: 10.1093/plphys/kiac118
• Publication Date: 2022-03-14T12:07:49Z
• Authors: Nian Wang; Beibei Zhang; Tian Yao; Chao Shen; Tianwang Wen; Ruiting Zhang; Yuanxue Li; Yu Le; Zhonghua Li; Xianlong Zhang; Zhongxu Lin
• Keywords: Anthocyanins; 0106 biological sciences; 0301 basic medicine; Gossypium; 03 medical and health sciences; Phenotype; Gene Expression Regulation, Plant; Gene Expression Profiling; Proanthocyanidins; Cotton Fiber; 01 natural sciences

Abstract Red foliated cotton is a typical dominant mutation trait in upland (Gossypium hirsutum). Although mutants have been described, few responsible genes identified and characterized. In this study, we performed map-based cloning of the red mutant gene (Re) derived from cross between G. hirsutum cv. Emian22 barbadense acc. 3–79. Through expression profiling, metabolic pathway analysis, sequencing candidate genes, Re was as an MYB113 transcription factor. A repeat sequence variation promoter region increased activity promoter, which enhanced Re. driven by 35S produced phenotype, expected. When fiber elongation-specific α-expansin 2 (PGbEXPA2), specifically expressed 5- to 10-day post-anthesis fibers rather than other tissues, resulting brown mature fibers. responded light through phytochrome-interacting factor 4 formed dimer with transparent testa 8, its well that anthocyanin synthase UDP-glucose:flavonoid 3-o-glucosyl transferase, thus activated entire metabolism pathway. Our research has cotton, paves way for detailed studies proanthocyanidin pigment accumulation provides alternative strategy producing fiber.