HEAT SHOCK PROTEIN 90.6 interacts with carbon and nitrogen metabolism components during seed development

• DOI: 10.1093/plphys/kiad019
• Publication Date: 2023-01-18T18:10:16Z
• Authors: Jianghai Xu; Zhijia Yang; Xiaohong Fei; Meiling Zhang; Yang Cui; Xiangbo Zhang; Kaiwen Tan; Lizhu E; Haiming Zhao; Jinsheng Lai; Qian Zhao; Weibin Song
• Keywords: Nitrogen Cycle; Protein metabolism

Abstract Carbon and nitrogen are the two main nutrients in maize (Zea mays L.) kernels, kernel filling metabolism determine seed formation germination. However, molecular mechanisms underlying relationship between corresponding carbon remain largely unknown. Here, we found that HEAT SHOCK PROTEIN 90.6 (HSP90.6) is involved both processes of nitrogen. A single-amino acid mutation within HATPase_c domain HSP90.6 led to small kernels. Transcriptome profiling showed expression amino biosynthesis- metabolism-related genes was significantly downregulated hsp90.6 mutant. Further evidence strong interactions 26S proteasome subunits REGULATORY PARTICLE NON-ATPASE6 (RPN6) PROTEASOME BETA SUBUNITD2 (PBD2). The reduced activity proteasome, resulting accumulation ubiquitinated proteins defects recycling. Moreover, verified through interacting with 14-3-3 protein GENERAL FACTOR14-4 (GF14-4). Collectively, our findings revealed development by components controlling metabolism.