Pulvinar slits: Cellulose-deficient and de-methyl-esterified pectin-rich structures in a legume motor cell

• DOI: 10.1093/plphys/kiad105
• Publication Date: 2023-02-28T00:56:05Z
• Authors: Masahiro Takahara; Satoru Tsugawa; Shingo Sakamoto; Taku Demura; Miyuki T Nakata
• Keywords: Turgor pressure; Pectin; Monosaccharide

Abstract The cortical motor cells (CMCs) in a legume pulvinus execute the reversible deformation leaf movement that is driven by changes turgor pressure. In contrast to underlying osmotic regulation property, cell wall structure of CMCs contributes has yet be characterized detail. Here, we report circumferential slits with low levels cellulose deposition, which are widely conserved among species. This unique and distinct from any other primary walls reported so far; thus, named them “pulvinar slits.” Notably, predominantly detected de-methyl-esterified homogalacturonan inside pulvinar slits, deposition highly methyl-esterified homogalacturonan, as cellulose. addition, Fourier transform infrared spectroscopy analysis indicated composition pulvini different axial organs, such petioles or stems. Moreover, monosaccharide showed pectin-rich organs like developing stems amount galacturonic acid greater than Computer modeling suggested facilitate anisotropic extension direction perpendicular presence When tissue slices were transferred extracellular conditions, altered their opening width, indicating deformability. this study, thus distinctive CMCs, adding our knowledge repetitive organ well structural diversity function plant wall.