Multi-copper oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic redox-based reactions in roots

• DOI: 10.1093/plphys/kiad207
• Publication Date: 2023-04-03T11:59:26Z
• Authors: Chaofan Chen; Yi Zhang; Jianfa Cai; Yuting Qiu; Lihong Li; Chengxu Gao; Yiqun Gao; Meiyu Ke; Shengwei Wu; Chuan Wei; Jiaomei Chen; Tongda Xu; Jiří Friml; Junqi Wang; Ruixi Li; Daiyin Chao; Baocai Zhang; Xu Chen; Zhen Gao
• Keywords: Endodermis; Epidermis (zoology); Cell Cortex

The primary cell wall is a fundamental plant constituent that flexible but sufficiently rigid to support the shape. Although many studies have demonstrated reactive oxygen species (ROS) serve as important signaling messengers modify structure and affect cellular growth, regulatory mechanism underlying spatial-temporal regulation of ROS activity for maintenance remains largely unclear. Here, we demonstrate role Arabidopsis (Arabidopsis thaliana) multicopper oxidase-like protein skewed 5 (SKU5) its homolog SKU5-similar 1 (SKS1) in root formation through modulating homeostasis. Loss SKU5 SKS1 function resulted aberrant division planes, protruding walls, ectopic deposition iron, reduced nicotinamide adeninedinucleotide phosphate (NADPH) oxidase-dependent overproduction epidermis-cortex cortex-endodermis junctions. A decrease level or inhibition NADPH oxidase rescued defects sku5 sks1 double mutants. proteins were activated by iron treatment, over-accumulated walls between epidermis cortex layers sks1. glycosylphosphatidylinositol-anchored motif was crucial membrane association functionality SKS1. Overall, our results identified regulators at surface growth.