Molecular Cloning and Expression of the Duplicated Thyroid Hormone Responsive Spot 14 (THRSP) Genes in Ducks

• DOI: 10.1093/ps/85.10.1746
• Publication Date: 2014-02-19T00:24:35Z
• Authors: K. Zhan; Z.C. Hou; H.F. Li; G.Y. Xu; R. Zhao; N. Yang
• Keywords: Cloning (programming)

Thyroid hormone responsive Spot 14 (THRSP) is suggested as a transcription factor involved in the regulation of adipogenic enzymes by 3 thyroid response elements promoter region. In chicken genome, THRSP gene was identified to duplicate into 2 paralogs, THRSPα and THRSPβ. current study, cDNA sequences duplicated duck genes were cloned real-time PCR rapid amplification ends. Duck THRSPβ predicted encode peptides with 133 amino acids, which had 74 68% sequence identity at level, 78 74% acid level counterparts, respectively. A high percentage (73.1%) G C nucleotides found 3′ untranslated region cDNA. Although low similarity peptide composition shared between ducks mammals, moderate chickens, many properties THRSP, including pI, subcellular localization functional domains seemed be highly conserved. The present study demonstrated that duplicates paralogs chickens. Phylogenetic analysis indicated duplication for appeared have taken place preceding chicken–duck split, diverging rate faster genome than genome. Expression quantitative showed more actively transcribed fat tissues (i.e., s.c. abdominal fat) liver, mRNA concentrations higher liver fat.