Development of Transgenic Chickens Expressing Human Parathormone Under the Control of a Ubiquitous Promoter by Using a Retrovirus Vector System

• DOI: 10.1093/ps/86.10.2221
• Publication Date: 2014-02-19T00:23:22Z
• Authors: S.H. Lee; M.K. Gupta; D.W. Han; S.Y. Han; S.J. Uhm; T. Kim; H.T. Lee
• Keywords: Rous sarcoma virus

Transgenic chickens, ubiquitously expressing a human protein, could be very useful model system for studying the role of proteins in embryonic development as well efficiently producing pharmaceutical drugs bioreactors. Human parathormone (hPTH) secreted from parathyroid glands plays significant calcium homeostasis and is an important therapeutic agent treatment osteoporosis humans. Here, by using robust replication-defective Moloney murine leukemia virus-based retrovirus vector encapsidated with vesicular stomatitis virus G glycoprotein, we generated transgenic chickens hPTH under control ubiquitous Rous sarcoma promoter. The recombinant was injected into subgerminal cavity freshly laid eggs at blastodermal stage. After 21 d incubation, 42 chicks hatched 473 retrovirus-injected eggs. All living were found to express vector-encoded gene diverse organs, revealed PCR reverse transcription-PCR analysis primer pairs specific hPTH. Four days after hatching, 6 died 14 showed phenotypic deformities. At 18 wk age, only 3 G0 survived. They also released hormone their blood transmitted G1 embryos. However, although embryos alive none hatched. An electrochemiluminescence immunoassay further that expression level markedly elevated mammalian cells infected vector. Thus, demonstrated protein promoter, not efficient bioreactor production drugs, but studies on development. To our knowledge, this first report promoter system.