Biological properties and characterization of several variations of a clinical human plasma-based skin substitute model and its manufacturing process

• DOI: 10.1093/rb/rbae115
• Publication Date: 2024-09-26T18:04:08Z
• Authors: Álvaro Sierra-Sán...; Jorge Cabañas-Pen...; Sandra Igual-Roger; Luis Martínez-Her...; Olga Espinosa-Ibáñez; Raquel Sanabria-d...; María I Quiñones-...; Ana Ubago-Rodríguez; Antonio Lizana-Mo...; Ana Fernández-Gon...; Jorge Guerrero-Calvo; Natividad Fernánd...; Arena Ramírez-Muñoz; Salvador Arias-Sa...
• Keywords: Characterization

Abstract Human plasma is a natural biomaterial that due to their protein composition widely used for the development of clinical products, especially in field dermatology. In this context, has been as scaffold alone or combined with others cellular human plasma-based skin substitutes (HPSSs). Herein, biological properties (cell viability, cell metabolic activity, secretion profile and histology) several variations HPSS model, regarding (alone six secondary biomaterials – serine, fibronectin, collagen, two types laminins hyaluronic acid), structure (trilayer, bilayer, monolayer control without cells) tissue origin (abdominal foreskin manufacturing process [effect partial dehydration viability comparison between submerged (SUB) air/liquid interface (ALI) methodologies] have evaluated compared. Results reveal use main determines vitro properties, rather than added. Moreover, characteristics are similar regardless cells (from abdomen foreskin). However, manufacture more complex (trilayer bilayer) demonstrated be better terms activity wound healing (bFGF, EGF, VEGF-A, CCL5) HPSSs, when ALI culture methodology applied. application dehydration, although required achieve an appropriate structure, reduce all cases. These data indicate model robust reliable subtypes here analysed could promising approaches depending on target dermatological disease.