Cytogenetic dose-response in vitro for biological dosimetry after exposure to high doses of gamma-rays

• DOI: 10.1093/rpd/ncs200
• Publication Date: 2012-08-25T00:46:22Z
• Authors: V. A. Vinnikov; N. A. Maznyk
• Keywords: Chromosome Aberrations; Male; Mitosis; Dose-Response Relationship, Radiation; Middle Aged; 03 medical and health sciences; 0302 clinical medicine; Gamma Rays; Cytogenetic Analysis; Humans; Female; Lymphocytes; Cobalt Radioisotopes; Radiometry; Cells, Cultured

The dose response for dicentrics plus centric rings and total unstable chromosome-type aberrations was studied in the first mitoses of cultured human peripheral blood lymphocytes irradiated in vitro to doses of ∼2, 4, 6, 8, 10, 16 and 20 Gy of acute (60)Со gamma-rays. A dose-dependent increase of aberration yield was accompanied by a tendency to the underdispersion of dicentrics and centric rings among cells distributions compared with Poisson statistics at doses ≥6 Gy. The formal fitting of the data to a linear-quadratic model resulted in an equation with the linear and quadratic coefficients ranged 0.098-0.129×cell(-1)×Gy(-1) and 0.039-0.034×cell(-1)×Gy(-2), respectively, depending on the fitting method. The actual radiation-induced aberration yield was markedly lower than expected from a calibration curve, generated earlier within a lower dose range. Interlaboratory variations in reported dicentric yields induced by medium-to-high radiation doses in vitro are discussed.