Sleep Deprivation During Early-Adult Development Results in Long-Lasting Learning Deficits in Adult Drosophila

• DOI: 10.1093/sleep/34.2.137
• Publication Date: 2017-02-05T16:31:52Z
• Authors: Laurent Seugnet; Yasuko Suzuki; Jeff M. Donlea; Laura Gottschalk; Paul J. Shaw
• Keywords: Sleep

Multiple lines of evidence indicate that sleep is important for the developing brain, although little known about which cellular and molecular pathways are affected. Thus, aim this study was to determine whether early adult life Drosophila, associated with high amounts critical periods brain plasticity, could be used as a model identify developmental processes require sleep.Wild type Canton-S Drosophila melanogaster. DESIGN;Flies were deprived on their first full day allowed recover undisturbed at least 3 days. The animals then tested short-term memory response-inhibition using aversive phototaxis suppression (APS). Components dopamine signaling further evaluated mRNA profiling, immunohistochemistry, pharmacological treatments.Flies exposed acute deprivation showed impairments in response inhibition persisted 6 These performance reversed by agonists, suggesting deficits consequence reduced signaling. However, did not impact dopaminergic neurons measured number or levels dopamine, pale (tyrosine hydroxylase), dopadecarboxylase, Dopamine transporter. impacted increased transcript receptors D2R dDA1. Importantly, blocking through dDA1 receptor during window prevented subsequent learning impairments.These data plays an phylogenetically conserved role brain.