Self-Assembled Matrigel-Free iPSC-Derived Liver Organoids Demonstrate Wide-Ranging Highly Differentiated Liver Functions

• DOI: 10.1093/stmcls/sxac090
• Publication Date: 2022-12-27T10:12:02Z
• Authors: Yun Weng; Simon Han; Maria T Sekyi; Tao Su; Aras N Mattis; Tammy T Chang
• Keywords: Organoid; Matrigel; Regenerative Medicine

Abstract Human induced pluripotent stem cell (iPSC)-derived liver organoids serve as models of organogenesis, disease, drug screening, and regenerative medicine. Prevailing methods for generating rely on Matrigel, whose batch-to-batch variability xenogeneic source pose challenges to mechanistic research translation human clinical therapy. In this report, we demonstrate that self-assembled Matrigel-free iPSC-derived developed in rotating wall vessels (RWVs) exhibit greater hepatocyte-specific functions than formed Matrigel. We show RWVs produce highly functional part by eliminating the need which has adverse effects hepatic lineage differentiation. RWV sustain durable function over long-term culture express a range mature genes at levels comparable adult liver, while retaining some fetal features. Our results indicate provide simple high-throughput way generate suitable applications.