Wood Smoke Particles Stimulate MUC5AC Overproduction by Human Bronchial Epithelial Cells Through TRPA1 and EGFR Signaling

• DOI: 10.1093/toxsci/kfaa006
• Publication Date: 2020-01-10T20:10:32Z
• Authors: Tosifa A Memon; Nam D Nguyen; Katherine L Burrell; Abigail F Scott; Marysol Almestica...; Emmanuel Rapp; Cassandra E Deeri...; Christopher A Reilly
• Keywords: 0301 basic medicine; Inhalation Exposure; 0303 health sciences; Glycogen Synthase Kinase 3 beta; Bronchi; Epithelial Cells; Mucin 5AC; Wood; p38 Mitogen-Activated Protein Kinases; Up-Regulation; 3. Good health; 12. Responsible consumption; ErbB Receptors; Mice, Inbred C57BL; 03 medical and health sciences; 13. Climate action; Smoke; 11. Sustainability; Animals; Humans; TRPA1 Cation Channel; Cells, Cultured; Signal Transduction

Abstract Mucus hypersecretion is a pathological feature of acute inflammatory and chronic obstructive pulmonary diseases. Exposure to air pollutants can be a cause of pathological mucus overproduction, but mechanisms by which different forms of air pollutants elicit this response are not fully understood. In this study, particulate matter (PM) generated from burning pine wood and other types of biomass was used to determine mechanisms by which these forms of PM stimulate mucin gene expression and secretion by primary human bronchial epithelial cells (HBECs). Biomass PM < 2.5 μm generated from pine wood and several other fuels stimulated the expression and secretion of the gel-forming glycoprotein MUC5AC by HBECs. Muc5ac gene induction was also observed in mouse airways following subacute oropharyngeal delivery of pine wood smoke PM. In HBECs, MUC5AC was also induced by the transient receptor potential ankyrin-1 (TRPA1) agonists’ coniferaldehyde, a component of pine smoke PM, and allyl isothiocyanate, and was attenuated by a TRPA1 antagonist. Additionally, inhibition of epidermal growth factor receptor (EGFR/ErbB1) and the EGFR signaling partners p38 MAPK and GSK3β also prevented MUC5AC overexpression. Collectively, our results suggest that activation of TRPA1 and EGFR, paired with alterations to p38 MAPK and GSK3β activity, plays a major role in MUC5AC overproduction by bronchial epithelial cells exposed to biomass smoke PM. These results reveal specific processes for how biomass smoke PM may impact the human respiratory system and highlight potential avenues for therapeutic manipulation of lung diseases that are affected by air pollutants.