Circ_0089282 inhibits carbon black nanoparticle-induced DNA damage by promoting DNA repair protein in the lung

• DOI: 10.1093/toxsci/kfad002
• Publication Date: 2023-01-08T03:22:43Z
• Authors: Han Zhang; Hanyu Zhou; Nan Zhang; Yangyang Jia; Miaoyun Qiu; Shuwei Yao; Xintong Chen; Lan Qiu; Saifeng Li; Yiguo Jiang; Yun Zhou
• Keywords: Carbon Nanoparticles

Inhalation of carbon black nanoparticles (CBNPs) can impair lung tissue and cause DNA damage, but the epigenetic mechanism responsible for these effects is still unclear. We explored role circular RNAs (circRNAs) in damage induced by CBNPs lung. Human bronchial epithelial cell lines (16HBE BEAS-2B) were treated with 0, 5, 10, 20, 40, or 80 μg/ml 24, 48, 72 h, BALB/c mice exposed to 8 μg/d 14 days establish vitro vivo models CBNP exposure, respectively. found that caused double-strand breaks Using high-throughput sequencing quantitative real-time PCR identify CBNP-related circRNAs, we identified a novel circRNA (circ_0089282) was overexpressed CBNP-exposed group. used gain-/loss-of-function approaches, RNA pulldown assays, silver staining explore regulatory function circ_0089282 its interactions targeted proteins. interference could increase CBNP-induced whereas overexpression resulted opposite. Circ_0089282 directly bind fused sarcoma (FUS) protein positively regulate downstream repair ligase 4 (LIG4) through FUS. This effect on via promotion LIG4 illustrated between genetics epigenetics toxicology.