Bisphenol A Exposure Induces Apoptosis and Upregulation of Fas/FasL and Caspase-3 Expression in the Testes of Mice

• DOI: 10.1093/toxsci/kfp024
• Publication Date: 2009-02-05T01:56:24Z
• Authors: Yuan-Jie Li; Tian-Bao Song; Yan-Yan Cai; Jin-Song Zhou; Xin Song; Xuan Zhao; Xiao-Lin Wu
• Keywords: Fas ligand; Xenoestrogen; Terminal deoxynucleotidyl transferase

There are controversies about adverse effects of bisphenol A (BPA), a ubiquitous xenoestrogen, on reproduction and development male animals. To understand BPA action assess its risk more completely, we examined the impact at high doses testes pubertal Kunming (China) mice. 0 (control), 160, 480, 960 mg/kg/day was given by gavage to mice from postnatal days (PND) 31-44, followed observation morphology detection apoptosis expressions Fas/FasL active caspase-3 PND 45, 60, 90 terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling, immunohistochemistry, Western blotting. no effect 160 mg/kg/day, however, 480 there underdevelopment disruption spermatogenesis. were many apoptotic Leydig germ cells in with indices being significantly increased compared controls. The expression Fas localized same cell types as occurred, levels Fas, FasL, disturbed spermatogenesis, upregulation persisted 90. results suggest that high-dose induces mouse testis through Fas-signaling pathway. Therefore, is concern reproductive health for humans occupationally exposed BPA.