Proteomic Analysis of Arsenic-Exposed Zebrafish (Danio rerio) Identifies Altered Expression in Proteins Involved in Fibrosis and Lipid Uptake in a Gender-Specific Manner

• DOI: 10.1093/toxsci/kft086
• Publication Date: 2013-04-10T00:37:05Z
• Authors: Patrick Carlson; David M. Smalley; Rebecca J. Van Be...
• Keywords: Danio; Arsenic toxicity; Sodium arsenite; Proteome

The zebrafish (Danio rerio) was used to investigate protein expression in the liver following arsenic exposure. Several disorders have been linked exposure, including cancer, diabetes, and cardiovascular disease. mechanisms of toxicity are poorly understood. Prior studies described altered gene expression, inflammation, mitogenic signaling acute or chronic exposure models. A proteomic approach employed arsenic-induced alteration proteome a 7-day 50 ppb sodium arsenite. Over 740 unique proteins were identified, with fewer than 2% showing differential expression. Molecular pathway analysis software identified lipid metabolism transport as potential molecular targets. Immunoblots confirm changes, whereas qPCR changes. Overall, 25 differentially expressed gender-specific manner, 11 males 14 females. Of these 25, single protein, hydroxysteroid dehydrogenase like 2, showed decreased both females These findings indicate that is changes presented here seem be most prevalent metabolic pathways, suggesting increase fibrosis accumulation uptake