Combination of Metagenomics and Culture-Based Methods to Study the Interaction Between Ochratoxin A and Gut Microbiota

• DOI: 10.1093/toxsci/kfu128
• Publication Date: 2014-06-28T05:12:13Z
• Authors: Mingzhang Guo; Kunlun Huang; Siyuan Chen; Xiaozhe Qi; Xiaoyun He; Wen-Hsing Cheng; Yunbo Luo; Kai Xia; Wentao Xu
• Keywords: Male; 0303 health sciences; Dose-Response Relationship, Drug; Microbiota; Ochratoxins; Rats, Inbred F344; Intestines; Feces; Lactobacillus; 03 medical and health sciences; RNA, Ribosomal, 16S; Animals; Chromatography, Thin Layer; Metagenomics

Gut microbiota represent an important bridge between environmental substances and host metabolism. Here we reported a comprehensive study of gut interaction with ochratoxin A (OTA), major food-contaminating mycotoxin, using the combination metagenomics culture-based methods. Rats were given OTA (0, 70, or 210 μg/kg body weight) by gavage fecal samples collected at day 0 28. Bacterial genomic DNA was extracted from both 16S rRNA shotgun sequencing (two main methods metagenomics) performed. The results indicated treatment decreased within-subject diversity microbiota, relative abundance Lactobacillus increased considerably. Changes in functional genes including signal transduction, carbohydrate transport, transposase, amino acid transport system, mismatch repair observed. To further understand biological sense Lactobacillus, selective medium used to isolate species samples, strain 99.8% similarity plantarum PFK2 obtained. Thin-layer chromatography showed that this could absorb but not degrade OTA, which agreement result no related degradation increased. In conclusion, can be new strategy intestinal toxicity toxins find applicable bacterial strains for detoxification. When it comes kind mycotoxin cause compositional changes are key genus detoxify vivo.