Acute Ozone-Induced Pulmonary and Systemic Metabolic Effects Are Diminished in Adrenalectomized Rats

• DOI: 10.1093/toxsci/kfv331
• Publication Date: 2016-02-14T22:08:02Z
• Authors: Desinia B. Miller; Samantha J. Snow; Mette C. Schladwe...; Judy E. Richards; Andrew J. Ghio; Allen D. Ledbetter; Urmila P. Kodavanti
• Keywords: Corticosterone

Acute ozone exposure increases circulating stress hormones and induces metabolic alterations in animals. We hypothesized that the increase of adrenal-derived is necessary for both ozone-induced effects lung injury. Male Wistar-Kyoto rats underwent bilateral adrenal demedullation (DEMED), total adrenalectomy (ADREX), or sham surgery (SHAM). After a 4 day recovery, were exposed to air (1 ppm), h/day 1 2 days responses assessed immediately postexposure. Circulating adrenaline levels dropped nearly zero DEMED ADREX relative SHAM. Corticosterone tended be low rats. Adrenalectomy air-exposed caused modest changes metabolites toxicity parameters. Ozone-induced hyperglycemia glucose intolerance markedly attenuated with complete reversal Ozone increased epinephrine corticosterone SHAM but not Free fatty acids (P = .15) branched-chain amino after Lung minute volume was affected by labored breathing less pronounced protein leakage neutrophilic inflammation reduced (ADREX > DEMED). Ozone-mediated decreases white blood cells observed demonstrate peripheral injury/inflammation are mediated through likely via activation response pathway.