Quorum-Sensing Systems in the Plant Growth-Promoting Bacterium Paraburkholderia phytofirmans PsJN Exhibit Cross-Regulation and Are Involved in Biofilm Formation

• DOI: 10.1094/mpmi-01-17-0008-r
• Publication Date: 2017-05-26T12:34:35Z
• Authors: Ana Zúñiga; Raúl A. Donoso; Daniela Ruiz; Gonzalo A. Ruz; Bernardo González
• Keywords: Homoserine

Quorum-sensing systems play important roles in host colonization and establishment of Burkholderiales species. Beneficial Paraburkholderia species share a conserved quorum-sensing (QS) system, designated BraI/R, that controls different phenotypes. In this context, the plant growth-promoting bacterium phytofirmans PsJN possesses two homoserine lactone QS BpI.1/R.1 BpI.2/R.2 (BraI/R-like system). The system was previously reported to be colonize produce beneficial effects Arabidopsis thaliana plants. Here, we analyzed temporal variations gene transcript levels wild-type strain colonizing roots. expression patterns showed relevant differences both compared with unplanted control treatment. data were used reconstruct regulatory network model P. PsJN, using Boolean model. Also, examined phenotypic traits genes involved systems, mutants synthases genes. We observed regulates biofilm formation production phenotype associated lower specific extracytoplasmic function sigma factor ecf26.1 (implicated formation) bpI.1 mutant strain.