Action of Multiple Cell Wall–Degrading Enzymes Is Required for Elicitation of Innate Immune Responses During Xanthomonas oryzae pv. oryzae Infection in Rice

• DOI: 10.1094/mpmi-02-16-0039-r
• Publication Date: 2016-06-06T16:08:13Z
• Authors: Lavanya Tayi; Roshan Maku; Hitendra Kumar Patel; Ramesh V. Sonti
• Keywords: Callose

Xanthomonas oryzae pv. secretes a number of plant cell wall-degrading enzymes (CWDEs) whose purified preparations induce defense responses in rice. These are suppressed by X. using type 3 secretion system (T3SS) effectors and mutant (T3SS(-)) is an inducer rice responses. We assessed the role individual CWDEs induction during infection, mutating them genetic background T3SS(-). mutated genes for five different secreted oryzae, including two cellulases (clsA cbsA), one xylanase (xyn), pectinase (pglA), esterase (lipA), singly T3SS(-) background. have demonstrated that, as compared with cbsA(-)T3SS(-), clsA(-)T3SS(-), xyn(-)T3SS(-) deficient immune such callose deposits programmed death. In comparison, lipA(-) pglA(-)T3SS(-) efficient host Overall, these results indicate that collective action oryzae-secreted ClsA, CbsA, Xyn proteins required infection.