HrpS Is a Global Regulator on Type III Secretion System (T3SS) and Non-T3SS Genes in Pseudomonas savastanoi pv. phaseolicola

• DOI: 10.1094/mpmi-02-18-0035-r
• Publication Date: 2018-05-02T15:38:09Z
• Authors: Jingru Wang; Xiaolong Shao; Yingchao Zhang; Yanan Zhu; Pan Yang; Jian Yuan; Tingting Wang; Chunyan Yin; Wei Wang; Sheng Chen; Haihua Liang; Xin Deng
• Keywords: Chromatin immunoprecipitation

The type III secretion system (T3SS) is the main machinery for Pseudomonas savastanoi and other gram-negative bacteria to invade plant cells. HrpR HrpS form a hetero-hexamer, which activates expression of HrpL, induces all T3SS genes by binding 'hrp box' in promoters. However, individual molecular mechanism or has not been fully understood. Through chromatin immunoprecipitation coupled high-throughput DNA sequencing, we found that HrpR, HrpS, HrpL had four, 47, 31 targets on genome, respectively. directly bound promoter regions group non-T3SS genes. independently regulated these hrpL deletion strain. Additionally, HrpS-binding motif (GTGCCAAA) was identified, verified electrophoretic mobility shift assay lux-reporter assay. also motility biofilm formation P. savastanoi. present study strongly suggests alone can work as global regulator both [Formula: see text] Copyright © 2018 Author(s). This an open-access article distributed under CC BY-NC-ND 4.0 International license .