Constitutive Expression of the Xylanase Inhibitor TAXI-III Delays Fusarium Head Blight Symptoms in Durum Wheat Transgenic Plants

• DOI: 10.1094/mpmi-04-13-0121-r
• Publication Date: 2013-08-14T14:29:41Z
• Authors: Ilaria Moscetti; Silvio Tundo; Michela Janni; Luca Sella; Katia Gazzetti; Alexandra Tauzin; Thierry Giardina; Stefania Masci; Francesco Favaron; Renato D'Ovidio
• Keywords: Bipolaris; Fungal pathogen

Cereals contain xylanase inhibitor (XI) proteins which inhibit microbial xylanases and are considered part of the defense mechanisms to counteract pathogens. Nevertheless, in planta evidence for this role has not been reported yet. Therefore, we produced a number transgenic plants constitutively overexpressing TAXI-III, member TAXI type XI that is induced by pathogen infection. Results showed TAXI-III endows wheat with new inhibition capacities. We also correctly secreted into apoplast possesses expected parameters against xylanases. The properties correlate significant delay Fusarium head blight disease symptoms caused graminearum but do significantly influence leaf spot Bipolaris sorokiniana. contrasting result can be due different capacity activity these two fungal These results provide, first time, clear involved plant pathogens show potential manipulate accumulation improve resistance F. graminearum.