The Genome of Peronospora belbahrii Reveals High Heterozygosity, a Low Number of Canonical Effectors, and TC-Rich Promoters

• DOI: 10.1094/mpmi-07-19-0211-r
• Publication Date: 2020-04-02T04:09:45Z
• Authors: Marco Thines; Rahul Sharma; Sander Y. A. Rode...; Anna Gogleva; Howard S. Judelson; Xiaojuan Xia; Johan van den Hoogen; Miloslav Kitner; Joël Klein; Manon Neilen; Dick de Ridder; Michael F. Seidl; Guido van den Ack...; Francine Govers; Sebastian Schornack; David J. Studholme
• Keywords: Obligate; Comparative Genomics

Along with Plasmopara destructor, Peronosopora belbahrii has arguably been the economically most important newly emerging downy mildew pathogen of past two decades. Originating from Africa, it started devastating basil production throughout world, likely due to distribution infested seed material. Here, we present genome this and results comparisons its genomic features other oomycetes. The assembly nuclear was around 35.4 Mbp in length, an N50 scaffold length 248 kbp L50 count 46. circular mitochondrial consisted 40.1 kbp. From repeat-masked genome, 9,049 protein-coding genes were predicted, out which 335 predicted have extracellular functions, representing smallest secretome so far found peronosporalean About 16% consists repetitive sequences, and, based on simple sequence repeat regions, provide a set microsatellites that could be used for population genetic studies P. belbahrii. undergone high degree convergent evolution obligate parasitic groups, reflecting biotrophic lifestyle. Features secretome, signaling networks, promoters are presented, some patterns hypothesized reflect host specificity Peronospora species. In addition, suggest presence additional virulence factors apart classical effector classes promising candidates future functional studies.