Analysis of the Involvement of an Inducible Arabidopsis RNA-Dependent RNA Polymerase in Antiviral Defense

• DOI: 10.1094/mpmi.2003.16.3.206
• Publication Date: 2007-05-11T09:25:20Z
• Authors: Diqiu Yu; Baofang Fan; Stuart A. MacFarlane; Zhixiang Chen
• Keywords: RNA Silencing; Cucumovirus; RNA virus

RNA-dependent RNA polymerases (RdRPs) have been implicated in posttranscriptional gene silencing (PTGS) and antiviral defense. An Arabidopsis RdRP (SDE1/SGS2) has previously shown to be required for transgene-induced PTGS but no general role On the other hand, we recently that transgenic tobacco deficient an inducible (NtRdRP1) activity became more susceptible both Tobacco mosaic virus Potato X. Thus, different RdRPs may distinct roles closely related In present study, analyzed of a newly identified (AtRdRP1) plant AtRdRP1 encodes structurally NtRdRP1 is also induced by salicylic acid treatment infection. A T-DNA insertion mutant isolated possible alterations response viral When infected to-bamovirus tobravirus, knockout accumulated higher persistent levels RNAs lower, inoculated upper, systemically leaves than did wild-type plants. These results suggest ortholog plays Examination short studies using vector harboring endogenous that, while not virus-induced PTGS, can apparently promote turnover