Comparative Analysis of Two Neighboring Conducive and Suppressive Soils Toward Plant Parasitism Caused by Phelipanche ramosa on Brassica napus

• DOI: 10.1094/pbiomes-12-23-0140-r
• Publication Date: 2024-11-15T15:33:26Z
• Authors: Lisa Martinez; Jean-Bernard Pouv...; Christophe Jestin; Gregory Montiel; Antoine Gravot; Solenne Berardocco; Nathalie Marnet; Alain Bouchereau; Erwan Delage; Philippe Simier; Lucie Poulin
• Keywords: Allelopathy

In Western France, rapeseed ( Brassica napus) cultivation faces substantial yield losses due to the root holoparasitic plant Phelipanche ramosa. However, recent observations have shown a reduction in parasitism within previously heavily infested fields. This study investigates two neighboring soils with distinct levels of parasitic infestation, considered suppressive and conducive. Using cocultivation system napus P. ramosa, we comprehensively examined rhizosphere exudates, attachment, soil microbiota. Our findings revealed that effectively reduced by impeding broomrape attachment development, as well inducing necrosis tubercles. effect was specific postattachment stages, leaving germination haustoriogenesis preattachment stages unaffected. Analysis microbial structures suggested suppression is predominantly fungal rather than bacterial origin. Correlation network analyses identified three groups amplicon sequence variants (ASVs) associated suppression. Notably, seven ASVs were inversely correlated attachments, only one ASV, Berkeleyomyces, necrotrophic fungus responsible for black rot, positively more abundant soil. demonstrates contrasting development on physicochemically similar soils, highlighting central role dynamics rhizosphere. These results provide valuable insights into mechanisms underlying soil-mediated offering potential strategies mitigating impact this holoparasite yields region.