Drought Stress Enhances Up-Regulation of Anthocyanin Biosynthesis inGrapevine leafroll-associated virus 3-Infected in vitro Grapevine (Vitis vinifera) Leaves

• DOI: 10.1094/pdis-01-17-0104-re
• Publication Date: 2017-05-23T20:14:06Z
• Authors: Zhen-Hua Cui; Wen-Lu Bi; Xin-Yi Hao; Peng-Min Li; Ying Duan; M. Andrew Walker; Yan Xu; Qiao-Chun Wang
• Keywords: Drought stress; Plant Physiology

Reddish-purple coloration on the leaf blades and downward rolling of margins are typical symptoms grapevine leafroll disease (GLD) in red-fruited cultivars. These attributed to expression genes encoding enzymes for anthocyanins synthesis, accumulation flavonoids diseased leaves. Drought has been proven accelerate development GLD virus-infected leaves grapevine. However, it is not known how drought affects nor anthocyanin biosynthesis altered. The present study used HPLC determine types levels anthocyanins, applied reverse transcription quantitative polymerase chain reaction (RT-qPCR) analyze synthesis. Plantlets Grapevine leafroll-associated virus 3 (GLRaV-3)-infected Vitis vinifera 'Cabernet Sauvignon' were grown vitro under PEG-induced stress. found no anthocyanin-related peaks healthy plantlets with or without stress, while 11 detected infected but significantly higher drought-stressed plantlets. Increased total compounds was related PEG highest level up-regulated gene GLRaV-3-infected Analyses variance correlation that GLRaV-3-infection key factor increased accumulation. This involved up-regulation two genes, MYBA1 UFGT, their further enhanced by