Characterization of a Powdery Mildew Resistance Gene in Wheat Breeding Line 10V-2 and Its Application in Marker-Assisted Selection

• DOI: 10.1094/pdis-02-17-0199-re
• Publication Date: 2017-11-28T14:09:07Z
• Authors: Pengtao Ma; Hongxing Xu; Yunfeng Xu; Liping Song; Shuoshuo Liang; Yuan Sheng; Guohao Han; Xiaotian Zhang; Diaoguo An
• Keywords: Blumeria graminis; Centimorgan; Bulked segregant analysis; Marker-Assisted Selection; Common wheat; Genetic distance; Genetic Analysis; Molecular marker

Powdery mildew, caused by Blumeria graminis f. sp. tritici, is a serious disease of wheat (Triticum aestivum L.) throughout the world. Host resistance most effective and preferred means for managing this disease. Line 10V-2, breeding line with superior agronomic performance, shows broad-spectrum seedling to powdery mildew. Genetic analysis demonstrated that its was controlled single dominant gene, tentatively designated Pm10V-2. This gene localized near documented Pm2 locus on chromosome 5DS using simple sequence repeat (SSR) marker Cfd81. To saturate map Pm10V-2, more markers were developed bulked segregant RNA-Seq. Two single-nucleotide polymorphism (SNP) (Swgi047 Swgi064), three expressed tag (Swgi007, Swgi029, Swgi038), one SSR (Swgi066) polymorphic between resistant susceptible bulks showed tightly linked Pm10V-2 gene. flanked new Swgi064 Swgi066 at genetic distances 0.4 1.2 centimorgans (cM), respectively, cosegregated Swgi007 Swgi038. The homologous Pm2a cloned from 10V-2 based recent study. Although completely identical reported Pm2a-related they did not cosegregate but separated distance 1.6 cM, indicating different When inoculated multiple B. tritici isolates, had significantly spectrum other mildew (Pm) genes or locus. Therefore, may be allele Pm2-linked use in marker-assisted selection (MAS) breeding, seven applicable MAS confirmed, including newly (Swgi029, Swgi038, Swgi064) present work. Using these markers, great number lines desirable performance selected crosses involving KM5016, which has been entered Regional trials Hebei Province, China.