Characterization of theColletotrichumSpecies Causing Anthracnose in Andean Blackberry in Colombia

• DOI: 10.1094/pdis-07-13-0752-re
• Publication Date: 2014-05-29T17:39:26Z
• Authors: Lucía Afanador-Ka...; Alonso González; Lederson Gañán; Juan Fernando Mejía; Nadya Cardona; Elizabeth Alvarez
• Keywords: Benomyl; Colletotrichum; Sensu

Since 1992, anthracnose of Andean blackberry (Rubus glaucus) has generated losses as high 40% for farmers in Colombia. In this study, our goal was to characterize 240 Colletotrichum isolates from eight areas These were evaluated according morphological characteristics, sensitivity benomyl, pathogenicity, and genetic variability. Identification the genus achieved by using species complex-specific polymerase chain reaction primers. A multilocus phylogeny approach used identify level with sequences ribosomal internal transcribed spacer region partial actin, β-tubulin 2, calmodulin, chitin synthase 1, glutamine synthetase, glyceraldehyde-3-phosphate dehydrogenase genes. Most identified gloeosporioides sensu lato, associated Castilla ecotype, showed highly aggressive. Isolates C. acutatum lato found mainly on Thornless resistant intermediate aggressiveness. Only three boninense lato. The included fructicola, kahawae subsp. ciggaro, godetiae, karstii, brassicicola, undetermined spp. This study is first report these blackberry.