A Laminarin-Based Formulation Protects Wheat Against Zymoseptoria tritici via Direct Antifungal Activity and Elicitation of Host Defense-Related Genes

• DOI: 10.1094/pdis-08-21-1675-re
• Publication Date: 2022-01-03T18:06:33Z
• Authors: Marlon C. de Borba; Aline C. Velho; Mateus B. de Freitas; Maxime Holvoet; Alessandra Maia-G...; Raymonde Baltenweck; Maryline Magnin-R...; Béatrice Randoux; Jean-Louis Hilbert; Philippe Reignault; Philippe Hugueney; Ali Siah; Marciel J. Stadnik
• Keywords: Laminarin; Phenylalanine ammonia-lyase; Pathosystem; Chitinase; Appressorium

The present study aimed to evaluate the potential of laminarin-based formulation Vacciplant protect and induce resistance in wheat against Zymoseptoria tritici, a major pathogen on this crop. Under greenhouse conditions, single foliar spraying product 2 days before inoculation with Z. tritici reduced disease severity pycnidium density by 42 45%, respectively. exhibited direct antifungal activity conidial germination both vitro planta. Moreover, it planta substomatal colonization as well formation treated leaves. Molecular investigations revealed that elicits but did not prime expression several genes related defense pathways, including phenylpropanoids (phenylalanine ammonia-lyase chalcone synthase), octadecanoids (lipoxygenase allene oxide pathogenesis-related proteins (β-1,3-endoglucanase chitinase). By contrast, modulate oxalate oxidase gene involved reactive oxygen species metabolism. Ultrahigh-performance liquid chromatography-mass spectrometry analysis indicated limited changes leaf metabolome after application noninoculated inoculated suggesting low metabolic cost associated induction plant resistance. This provides evidence confers protection through elicitation defense-associated genes.