Four antimicrobial compounds and ISR induction are involved in biocontrol of crown gall disease by the plant beneficial rhizobacterium Bacillus velezensis FZB42

• DOI: 10.1094/pdis-10-24-2085-re
• Publication Date: 2025-02-21T07:27:06Z
• Authors: Xu Qin; Yueting Xiao; Qin Xiong; Wei-Liang Kong; Rainer Borriss; Zhaoliang Gao; Ben Fan
• Keywords: Surfactin; Jasmonic acid; Antibiosis

Crown gall disease (CGD), which is caused by the bacterium Agrobacterium, a common plant that often results in significant economic losses. Biocontrol offers an environmentally friendly and sustainable method to control CGD. In this study, we investigated biocontrol effect mechanism of CGD Japanese cherry trees B. velezensis FZB42, prototype strain growth promoting rhizobacteria (PGPR). We found FZB42 mutants unable produce one or combination four antimicrobial compounds (surfactin, macrolactin, bacilysin, difficidin) significantly reduced their inhibitory activities against A. tumefaciens XYT-58 ability controlling occurrence Using purified compounds, further demonstrated bacilysin surfactin can inhibit vitro. Inoculation with increased expression genes related jasmonic acid (JA), ethylene (ET), salicylic (SA) pathways seedlings when they were infected XYT-58. To our knowledge, first report difficidin also suppress Agrobacterium. Furthermore, indicate multiple induced systemic resistance (ISR) are involved velezensis. The elucidated here provides guidance for development application type PGPR