Potential for Seed Transmission of Verticillium longisporum in Oilseed Rape (Brassica napus)

• DOI: 10.1094/pdis-11-18-2024-re
• Publication Date: 2019-01-29T14:23:19Z
• Authors: Xiaorong Zheng; Daniel Teshome Lo...; Alice Bisola Eseola; Birger Koopmann; Andreas von Tiede...
• Keywords: Verticillium

Verticillium longisporum is a soilborne vascular fungal pathogen that has spread throughout the European oilseed rape cultivation area since 1980s and was detected in canola fields Canada 2014. In series of greenhouse field inoculation experiments using V. longisporum-resistant susceptible cultivars winter spring types rape, present study investigated potential dissemination by seeds Brassica napus. Greenhouse studies with DsRed-labeled isolate confirmed systemic growth from roots to seeds. Further monitoring plant colonization species-specific real-time polymerase chain reaction assay verified stem bases, pods, root-inoculated plants. The frequency recovery viable colonies harvested greenhouse-grown inoculated plants ranged 0.08 13.3%. seed transmission differed varying susceptibility longisporum. Subsequent on disease into offspring revealed only 1.7 2.3% showed symptoms as formation microsclerotia stems. Results field-grown studies. degree dependent crop type. Although low concentrations DNA were detectable severely infected significantly greater found spring-type at similar soil conditions inoculum densities. Correspondingly, plating agar yielded but not winter-type Lack infection two seasons. Equally, none grown diseased developed striping. results suggest rate depends colonization, which faster under than spring-sown compared an autumn-sown crop. According our studies, production cannot be confirmed.