First Report and Partial Molecular Characterization of a New Begomovirus Associated with Pigeon Pea in Puerto Rico

• DOI: 10.1094/pdis.2001.85.10.1119c
• Publication Date: 2007-05-11T10:04:33Z
• Authors: R. L. Rodriguez; S. Hong; D. P. Maxwell
• Keywords: Cajanus; Mosaic virus

Pigeon pea (Cajanus cajan (L.) Millsp.) is an important edible legume crop in Puerto Rico. In late fall 1997, symptoms of a golden mosaic virus were observed pigeon planting the municipality Villalba. The resembled those incited by Rhynchosia virus, whitefly-transmitted (1). Tests with 3F7 antigeminivirus antibody from Agdia (Elkhart, IN) confirmed presence begomovirus these symptomatic peas. Extraction DNA dried foliar tissue was accomplished methods described Rojas et al. (2), and subsequent viral amplification coat protein (CP) gene polymerase chain reaction primer pair AV494-AC1048 (3). A 550-bp fragment cloned sequenced (GenBank Accession No. AY028308). For sequence analysis, BLAST program at National Institutes Health used. first match 91% CP (Accession AF070924) Clitoria falcata next three matches approximately 86% (accession no. AF058024) Macroptilium lathyroides Rico, Dicliptera yellow mottle AF139168) Florida, Tobacco apical stunt AF076855) Mexico. On basis analysis this conserved region gene, it concluded that report new thus named virus. only for sp. GenBank Honduras AF239671). comparisons, not isolate Honduran References: (1) J. Bird 1975. Pages 3–25 in: Tropical Diseases Legumes. K. Maramorosch, eds. Academic Press, NY. (2) M. R. Plant Dis. 77:340, 1993. (3) S. D. Wyatt Brown. Phytopathology. 86:1288, 1996.