Virion-Associated Nucleic Acid-Based Metagenomics: A Decade of Advances in Molecular Characterization of Plant Viruses

• DOI: 10.1094/phyto-03-22-0096-rvw
• Publication Date: 2022-06-20T01:06:30Z
• Authors: Oumaima Moubset; Sarah François; François Maclot; Essowè Palanga; Charlotte Julian; Lisa Claude; Emmanuel Fernandez; Philippe Rott; Jean-Heinrich Dau...; Aymeric Antoine-L...; Pauline Bernardo; Arnaud G. Blouin; Coline Temple; Simona Kraberger; Rafaela S. Fontenele; Gordon W. Harkins; Yuxin Ma; Armelle Marais; Thierry Candresse; Sélim Ben Chéhida; Pierre Lefeuvre; Jean-Michel Lett; Arvind Varsani; Sébastien Massart; Mylène Ogliastro; Darren P. Martin; Denis Filloux; Philippe Roumagnac
• Keywords: 0301 basic medicine; 570; Viral RNA Silencing; [SDV]Life Sciences [q-bio]; Plant virus; [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]; Plant Science; virus des végétaux; Gene; génomique; Plant Viruses; Agricultural and Biological Sciences; Computational biology; 03 medical and health sciences; Endocrinology; http://aims.fao.org/aos/agrovoc/c_5985; Nucleic Acids; Biochemistry, Genetics and Molecular Biology; Virology; Genetics; Mycoviruses in Fungal Symbiosis and Pathogenesis; Biology; Ecosystem; H20 - Maladies des plantes; Plant Diseases; Ecology; Virion; Human virome; Life Sciences; Plants; http://aims.fao.org/aos/agrovoc/c_92382; Virus; [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology; http://aims.fao.org/aos/agrovoc/c_3791; FOS: Biological sciences; [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology; Viral RNA Silencing and Plant Immunity; identification; Metagenomics; Pathogen Detection; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; Genetics and Epidemiology of Plant Pathogens

Over the last decade, viral metagenomic studies have resulted in discovery of thousands previously unknown viruses. These are likely to play a pivotal role obtaining an accurate and robust understanding how viruses affect stability productivity ecosystems. Among metagenomics-based approaches that been developed since beginning 21st century, shotgun metagenomics applied specifically virion-associated nucleic acids (VANA) has used disentangle diversity world. We summarize herein results 24 VANA-based studies, focusing on plant insect samples conducted over decade (2010 2020). Collectively, from 85 different families were reliably detected these including capsidless RNA replicate fungi, oomycetes, plants. Finally, strengths weaknesses VANA approach summarized perspectives applications detection, epidemiological surveillance, environmental monitoring, ecology provided. [Formula: see text] Copyright © 2022 The Author(s). This is open access article distributed under CC BY-NC-ND 4.0 International license .