A Pseudomonas syringae Diversity Survey Reveals a Differentiated Phylotype of the Pathovar syringae Associated with the Mango Host and Mangotoxin Production

• DOI: 10.1094/phyto-04-13-0093-r
• Publication Date: 2013-10-08T19:07:13Z
• Authors: José A. Gutiérrez...; Víctor J. Carrión; Jesús Murillo; Eva Arrebola; Dawn L. Arnold; Francisco M. Cazorla; Antonio de Vicente
• Keywords: Pseudomonas syringae; Pathovar; Phylotype; Multilocus sequence typing

Pseudomonas syringae pv. syringae, the causal agent of bacterial apical necrosis (BAN) in mango crops, has been isolated different mango-producing areas worldwide. An extensive collection 87 P. strains from trees affected by BAN countries, but mainly Southern Spain, were initially examined repetitive sequence-based polymerase chain reaction (rep-PCR) to analyze genetic diversity with an epidemiological aim. rep-PCR was powerful assessing intrapathovar distribution and also allowing clustering mango, depending on isolation area. A clear pattern observed for all distinct other hosts, including same geographical regions as isolates. For this reason, a representative group 51 well some pathovars, further characterized determine their possible genetic, phenotypic, phylogenetic relationships. Similar results, randomly amplified polymorphic DNA PCR (RAPD-PCR) catabolic analysis using Biolog GN2 profile grouped 90% isolates together unique cluster. Interestingly, majority produced mangotoxin. The multilocus sequence typing strongly supports existence differentiated phylotype pathovar associated host mangotoxin production.