T6SS and T4SS Redundantly Secrete Effectors to Govern the Virulence and Bacterial Competition in Pectobacterium PccS1

• DOI: 10.1094/phyto-11-23-0455-r
• Publication Date: 2024-05-15T21:44:21Z
• Authors: Shu Che; Chen Sun; Liuke Yang; Min Zhou; Lingyan Xia; Jingyuan Yan; Mengyi Jiang; Jiaju Wang; Huan Wang; Wenjun Zhao; Ian Toth; Baishi Hu; Tao Guo; Jiaqin Fan
• Keywords: 0301 basic medicine; 570; 0303 health sciences; Virulence; Virulence Factors; Pectobacterium; 610; Type VI Secretion Systems; Type IV Secretion Systems; Bacterial Proteins; Glucans; Plant Diseases

Previous studies revealed that the type VI secretion system (T6SS) has an essential role in bacterial competition and virulence in many gram-negative bacteria. However, the role of T6SS in virulence in Pectobacterium atrosepticum remains controversial. We examined a closely related strain, PccS1, and discovered that its T6SS comprises a single-copy cluster of 17 core genes with a higher identity to homologs from P. atrosepticum. Through extensive phenotypic and functional analyses of over 220 derivatives of PccS1, we found that three of the five VgrGs could be classified into group I VgrGs. These VgrGs interacted with corresponding DUF4123 domain proteins, which were secreted outside of the membrane and were dependent on either the T6SS or type IV secretion system (T4SS). This interaction directly governed virulence and competition. Meanwhile, supernatant proteomic analyses with strains defective in the T6SS and/or T4SS confirmed that effectors, such as FhaB, were secreted redundantly to control the virulence and suppress host callose deposition in the course of infection. Notably, this redundant secretion mechanism between the T6SS and T4SS is believed to be the first of its kind in bacteria.