Genome-Enabled Phylogeographic Investigation of the Quarantine Pathogen Ralstonia solanacearum Race 3 Biovar 2 and Screening for Sources of Resistance Against Its Core Effectors

• DOI: 10.1094/phyto-12-14-0373-r
• Publication Date: 2015-02-24T20:03:16Z
• Authors: Christopher R. Cl...; David J. Studholme; Byron Hayes; Brendan Runde; Alexandra Weisberg; Rongman Cai; Tadeusz Wroblewski; Marie-Christine D...; Emmanuel Wicker; Jose A. Castillo; Boris A. Vinatzer
• Keywords: Ralstonia solanacearum; Biovar; Bacterial wilt; R gene

Phylogeographic studies inform about routes of pathogen dissemination and are instrumental for improving import/export controls. Genomes 17 isolates the bacterial wilt potato brown rot Ralstonia solanacearum race 3 biovar 2 (R3bv2), a Select Agent in United States, were thus analyzed to get insight into phylogeography this pathogen. Thirteen fourteen from Europe, Africa, Asia found belong single clonal lineage while South America genetically diverse tended carry ancestral alleles at genomic loci consistent with American origin R3bv2. The R3bv2 share core repertoire 31 type III-secreted effector genes representing excellent candidates be targeted resistance breeding programs develop durable disease resistance. Toward goal, 27 effectors tested eggplant, tomato, pepper, tobacco, lettuce induction hypersensitive-like response indicative recognition by cognate receptors. Fifteen effectors, eight them triggered one or more plant species. These genotypes may harbor that could identified mapped, cloned, expressed tomato potato, which sources genetic extremely limited.