Host Range and Characterization ofSunflower mosaic virus

• DOI: 10.1094/phyto.2002.92.7.694
• Publication Date: 2007-05-11T11:16:50Z
• Authors: T. J. Gulya; P. J. Shiel; T. Freeman; R. L. Jordan; T. Isakeit; P. H. Berger
• Keywords: Potyviridae; Tobacco etch virus; Helianthus; Helianthus annuus; Soybean mosaic virus; Mosaic virus

ABSTRACT Sunflower mosaic is caused by a putative member of the family Potyviridae. virus (SuMV) was characterized in terms host range, physical and biological characteristics, partial nucleotide amino acid sequence. Cells infected with SuMV had cytoplasmic inclusion bodies typical potyviruses. Of 74 genera tested, only species Helianthus, Sanvitalia, Zinnia, all Asteraceae, were systemic hosts. Commercial sunflower hybrids from United States, Europe, South Africa equally susceptible. The mean length purified particles approximately 723 nm. transmitted Myzus persicae Capitphorus elaegni, also seedborne at least one cultivar. Indirect enzyme-linked immunosorbent assay tests broad-spectrum potyvirus monoclonal antibody strongly positive. SuMV-specific polyclonal antisera recognized and, to lesser extent, Tobacco etch (TEV). When tested against panel 31 potyvirus-differentiating antibodies, distinct any previously tested. shared four epitopes TEV, but reaction profile more similar Tulip breaking (TBV). did not possess unique TBV. predicted coat protein molecular weight 30.5 kDa. 3' end genome cloned sequenced. Phylogenetic analysis sequence revealed that within Potyviridae, most closely related TEV.