Spatial Patterning of Scleral Papillae in the Embryonic Chicken Eye

• DOI: 10.1096/fasebj.2018.32.1_supplement.776.2
• Publication Date: 2021-06-21T16:42:55Z
• Authors: Jennifer L. Giffin; Molly Hayes; Daniel DT. Andrews; Nicholas W. Zinck; Tamara A. Franz‐O...
• Keywords: Sclera

The anterior portion of the eye in birds is supported by a ring bony elements within sclera, constituting scleral ossicles. These intramembranous bones are induced series transient papillae overlying conjunctival epithelium during embryonic development. A well‐defined spatiotemporal pattern formation followed, resulting complete circle 13–16 evenly‐spaced per chicken embryo. regular distribution and variable number this system give it strong similarity to other reaction‐diffusion models patterning, such as feather carapace formation, which short‐range activation coupled with long‐range inhibition. Therefore, objective study investigate whether formed through mechanism eye. To carry out study, embryos at Hamburger Hamilton stages 28–34 (5.5–8 days post fertilization) were collected, corresponding induction set papillae. Quantitative morphometrical analyses performed on dissected eyes, confirming that while size increases throughout development, spacing between does not. Computer modelling using Meinhardt‐Gierer equation indicates development likely follows typical Turing formation. PCR discrete papillary interpapillary regions will enable us interactions known signalling molecules including Hedgehog (Hh), Bone Morphogenic Proteins (BMPs), Fibroblast Growth Factors (FGFs) Wingless (Wnt). results provide considerable insight into explains ability maintain their even regardless differences numbers among species or left right eyes same individual. Identifying pathways implicated would further enhance our understanding not only position, but also shape ossicles compensation for gaps sclerotic occur. Support Funding Information This research was funded Natural Sciences Engineering Research Council Canada. abstract from Experimental Biology 2018 Meeting. There no full text article associated published FASEB Journal .