Extracts of Two Relatively Safe South African Alien Weeds have Synergistic Activity with Conventional Antibiotics against some Urinary Tract Pathogens

• DOI: 10.1096/fasebj.2019.33.1_supplement.504.5
• Publication Date: 2021-06-22T00:40:57Z
• Authors: Aitebiremen Gift ...; Johannes Van Staden; Lyndy Joy McGaw
• Keywords: ABTS; Enterococcus faecalis

Plants have potential to play a role in the treatment of urinary tract infections, as they contain important bioactive components. These components may act synergism with conventional antibiotics which are presently under threat microbial resistance. They also key reduction oxidative stress. Synergistic activity dichloromethane, acetone, 70% ethanol, 50% methanol and hot water extracts southern African alien weeds Chromolaena odorata Tithonia rotundifolia combined gentamicin amphotericin B was studied using checkerboard method. Antioxidant determined quantitative 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging 2,2′‐azinobis‐(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) decolorisation methods. The anti‐inflammatory through 15‐lipoxygense enzyme assay. Toxicity screening carried out against Vero monkey kidney, human carcinoma Caco‐2 liver C3A cell lines colorimetric additive effects were observed among all tested combination strains Escherichia coli , Enterococcus faecalis Klebsiella pneumoniae Salmonella Typhimurium, Aspergillus fumigatus Candida albicans . Although both plant species had antioxidant activity, C. displayed better ABTS assay ethanol extract showing inhibitory concentration (IC 50 ) 1.96 μg/mL, than that vitamin C = 2.027 μg/mL). dichloromethane T. DPPH an IC 16.231 μg/mL. All positive control quercetin 24.601 μg/mL) except for acetone slightly higher 26.098) control. Lethal concentrations (LC Vero, cells ranged from 0.78 0.96mg/mL, 0.198 0.32mg/mL 0.67 0.88 mg/mL respectively, indicating relatively safe vitro Results this study indicate these weedy comparatively non‐cytotoxic compounds synergy infections. Additionally reduce stress inflammation. Further studies determine active component(s) weed plants ongoing. Support or Funding Information No funding This abstract is Experimental Biology 2019 Meeting. There no full text article associated published FASEB Journal