The Immunological Response to Chronic Stress in Obese Mice

• DOI: 10.1096/fasebj.2019.33.1_supplement.581.3
• Publication Date: 2021-06-22T00:43:36Z
• Authors: Matthew Parsley; Emily Burrage; Kent D Marshall; Garrett D Clemons; Abigail Tice; Randy W Bryner; Kathleen Brundage; Eric E Kelley; Paul D Chantler
• Keywords: Chronic Stress

Chronic stress is a major risk factor for cardiovascular disease. We have shown that chronic stress, which acts as trigger anxiety and depression, significantly reduces vascular function in obese rats mice. Such dysfunction linked to an increase systemic inflammation. The spleen adipose tissue are sources of these peripheral immune cells. sought identify the immunological changes after unpredictable (UCMS). Spleen gonadal cells from male mice who underwent either 8 weeks non‐UCMS (controls, n=6) or UCMS (n=6) 5 days/week/7Hrs/day elicit depressive phenotype were analyzed by flow cytometry. size was 23% lower (p=0.08) group vs. controls, yet body weight reduced compared controls (48±0.7 42±0.8, p<0.01), resulting no differences ratio spleen/body weight. To evaluate quantify cellular composition with UCMS, we harvested spleens control tended numbers CD45 controls. Although CD8, CD4 CD4/CD8 ratio, B‐cells noted, number M2 macrophages generally group, M1 macrophages. In visceral tissue, CD45+ accompanied CD8+ CD4+ groups. Our data suggests alters cell populations fat Given tissues play important role release into circulation, could on noted our exposure stress. Support Funding Information OD016165 (KB) This abstract Experimental Biology 2019 Meeting. There full text article associated this published FASEB Journal .