Pentadecapeptide BPC 157 Therapy in Rats with Cysteamine Induced‐Terminal Ileitis

• DOI: 10.1096/fasebj.2020.34.s1.03908
• Publication Date: 2020-04-21T00:30:00Z
• Authors: Bozo Radic; Mariam Samara; Helena Zizek; Slaven Gojkovic; Ivan Krezic; Dominik Malekinusic; Tajana Durasin; Domagoj Drmic; Fedor Amic; Andrea Petrovic; Katarina Horvat P...; Sven Seiwerth; Predrag Sikiric
• Keywords: Cysteamine; Ileitis

Aim We introduce pentadecapeptide BPC 157 therapy in rats with cysteamine induced‐terminal ileitis, 1 h, month, 2 months. counteracted gross hyperemia, edema, erosion, bleeding, and, microscopically, significant loss of villous architecture, and shortening villae severe lymphocytic infiltrate. counteracts various lesions the whole GI‐tract free radicals formation (Curr Pharm Des 2018;24:1990–2001), tested ulcerative colitis trials now multiple sclerosis 2018;24:1990–2001). Previously, cysteamine, was known to induce gastric acid hypersecretion, as a prototype duodenal lesion (Lancet 1979 Oct 27;2(8148):880–2). Subsequently, induced after gastrectomy, applied an enema, rats. all these (Life Sci 1994;54(5):PL63‐8, Dig Dis 1997 May;42(5):1029–37, J Physiol Paris 2001 Jan–Dec;95(1–6):283–8, Jan–Dec;95(1–6):261–70, Eur Pharmacol 2003 Sep 5;477(1):73–80, 2013 Oct;64(5):597–612) Methods Cysteamine (400 mg/kg, ml/rat) female Albino Wistar rats, 200 g bw into terminal ileum, 5 cm segment up ileocecal valve, which kept gently compressed for min, then released. Medication (/kg) (BPC (10 μg, 10 ng), or saline (5 ml) (controls)) abdominal bath immediately end application procedure, if were not sacrificed at continuously, perorally drinking water (0.16 μg/ml, 0.16 ng/ml, 12 ml/rat/day) till Results For assessment, erosion bleeding scores (0–3) summarized (giving 0–12 scoring, assessed Min/Med/Max). Control exhibit following scoring: 11/11/12 8/8/9 Contrarily, present markedly lower scoring (3/3/4 0/1/1 months, μg regimen Min/Med/Max, P<0.05, vs. control, least, while ng shows similar beneficial effect). Microscopically, much like patients, ileitis goes submucosal congestion, villae, lamina propria infiltrated mild infiltrate intraepithelial lymphocytes infiltration some epithelial elevation from propria. better preservation mucosal architecture appears treated There is only edema capillary congestion No found (Fig. , Fig. 2, left right 157). Conclusion further therapy, effect cysteamine‐terminal ileitis. Support Funding Information This work supported by University Zagreb, Croatia (Grant BM 099). Terminal Ileitis, hour Figure